1N9R
Crystal structure of a heptameric ring complex of yeast SmF in spacegroup P4122
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | AREA DETECTOR |
| Wavelength(s) | 0.9393 |
| Spacegroup name | P 41 2 2 |
| Unit cell lengths | 79.891, 79.891, 251.188 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.800 |
| R-factor | 0.25431 |
| Rwork | 0.254 |
| R-free | 0.26700 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1I81 truncated to poly-serine |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.100 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 79.000 | 2.950 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.066 | 0.390 |
| <I/σ(I)> | 19.6 | 4.6 |
| Completeness [%] | 99.9 | 99.9 * |
| Redundancy | 6.8 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 289 | PEG 3350, sodium acetate, pH 8.5, VAPOR DIFFUSION, SITTING DROP at 289K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Tris-NaCl | ||
| 2 | 1 | drop | dithiothreitol | 10 (mM) | |
| 3 | 1 | drop | protein | 20 (mg/ml) | |
| 4 | 1 | reservoir | PEG3350 | 24 (%) | |
| 5 | 1 | reservoir | Tris | 0.1 (M) | pH8.5 |
| 6 | 1 | reservoir | sodium acetate | 0.1 (M) |
