1N9P

Crystal Structure of the Cytoplasmic Domain of G-protein Activated Inward Rectifier Potassium Channel 1

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F2
Synchrotron site	CHESS
Beamline	F2
Temperature [K]	100
Detector technology	CCD
Collection date	2002-08-11
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9792, 0.9790, 0.9640
Spacegroup name	P 4 21 2
Unit cell lengths	76.020, 76.020, 86.110
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 1.800
R-factor	0.232
Rwork	0.231
R-free	0.25300
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	1.360 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.860
High resolution limit [Å]	1.800	1.800
Rmerge	0.057 *	0.174 *
Number of reflections	23971
<I/σ(I)>	20.2	12.9
Completeness [%]	99.5	96.6
Redundancy	19.1	13.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	4 *	DTT, HEPES, magnesium acetate, PEG 400, sodium chloride, Tris-HCl, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir	magnesium acetate	50 (mM)
3	1	reservoir	HEPES	50 (mM)	pH7.0
4	1	reservoir	PEG400	55 (%)