1N9P
Crystal Structure of the Cytoplasmic Domain of G-protein Activated Inward Rectifier Potassium Channel 1
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F2 |
| Synchrotron site | CHESS |
| Beamline | F2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-08-11 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9792, 0.9790, 0.9640 |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 76.020, 76.020, 86.110 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.800 |
| R-factor | 0.232 |
| Rwork | 0.231 |
| R-free | 0.25300 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.360 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.057 * | 0.174 * |
| Number of reflections | 23971 | |
| <I/σ(I)> | 20.2 | 12.9 |
| Completeness [%] | 99.5 | 96.6 |
| Redundancy | 19.1 | 13.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 4 * | DTT, HEPES, magnesium acetate, PEG 400, sodium chloride, Tris-HCl, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | reservoir | magnesium acetate | 50 (mM) | |
| 3 | 1 | reservoir | HEPES | 50 (mM) | pH7.0 |
| 4 | 1 | reservoir | PEG400 | 55 (%) |
