1N9O
Crystal structure of the Phot-LOV1 domain from Chlamydomonas reinhardtii in illuminated state. Composite data set.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-03-05 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.969 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 121.900, 121.900, 45.500 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 15.000 - 2.800 |
R-factor | 0.252 |
Rwork | 0.250 |
R-free | 0.29800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1g28 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.320 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | AMoRE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.000 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.129 * | 0.322 * |
Number of reflections | 5009 | |
<I/σ(I)> | 7.5 | 2.6 |
Completeness [%] | 95.2 | 82.5 |
Redundancy | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 20 * | HEPES, ammonium sulfate, PEG8000, pH 7.3, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 20 * | HEPES, ammonium sulfate, PEG8000, pH 7.3, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 20 * | HEPES, ammonium sulfate, PEG8000, pH 7.3, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10.5 (mg/ml) | |
2 | 1 | drop | sodium phosphate | 10 (mM) | |
3 | 1 | drop | 10 (mM) | pH8.0 | |
4 | 1 | reservoir | sodium HEPES | 100 (mM) | pH7.3 |
5 | 1 | reservoir | ammonium sulfate | 1.5-1.8 (M) | |
6 | 1 | reservoir | PEG8000 | 1-8 (%) |