1N9L
Crystal structure of the Phot-LOV1 domain from Chlamydomonas reinhardtii in the dark state.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-03-01 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9393 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 122.100, 122.100, 46.100 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 15.000 - 1.900 |
| R-factor | 0.233 |
| Rwork | 0.232 |
| R-free | 0.24800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1g28 |
| RMSD bond length | 0.008 * |
| RMSD bond angle | 1.280 * |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.068 * | 0.351 * |
| Total number of observations | 77830 * | |
| Number of reflections | 16142 | |
| <I/σ(I)> | 12.8 | 3.5 |
| Completeness [%] | 98.0 | 96.6 |
| Redundancy | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 20 * | HEPES, ammonium sulfate, PEG8000, pH 7.3, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10.5 (mg/ml) | |
| 2 | 1 | drop | sodium phosphate | 10 (mM) | |
| 3 | 1 | drop | 10 (mM) | pH8.0 | |
| 4 | 1 | reservoir | sodium HEPES | 100 (mM) | pH7.3 |
| 5 | 1 | reservoir | ammonium sulfate | 1.5-1.8 (M) | |
| 6 | 1 | reservoir | PEG8000 | 1-8 (%) |
