1N9F
Structure of earth-grown oxidized Myoglobin mutant YQR (ISS6A)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-07-27 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.001 |
| Spacegroup name | P 6 |
| Unit cell lengths | 90.542, 90.542, 45.243 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 15.000 - 1.800 |
| Rwork | 0.179 |
| R-free | 0.20200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1f65 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.890 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 1.930 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.089 | 0.207 |
| Number of reflections | 21977 | |
| <I/σ(I)> | 15.6 | 10.2 |
| Completeness [%] | 98.5 | 86.7 |
| Redundancy | 3.2 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 8.7 | 294 | Ammonium sulfate, tris, EDTA, pH 8.7, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | reservoir | Tris-HCl | 100 (mM) | pH8.7 |
| 3 | 1 | reservoir | EDTA | 1 (mM) | |
| 4 | 1 | reservoir | ammonium sulfate | 2.8 (M) |
