1N99
CRYSTAL STRUCTURE OF THE PDZ TANDEM OF HUMAN SYNTENIN
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X9B |
Synchrotron site | NSLS |
Beamline | X9B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-10-18 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.97946, 0.97133, 0.97900 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 100.727, 48.678, 74.690 |
Unit cell angles | 90.00, 120.82, 90.00 |
Refinement procedure
Resolution | 30.000 - 1.940 |
R-factor | 0.18641 |
Rwork | 0.184 |
R-free | 0.22700 * |
Structure solution method | MAD |
RMSD bond length | 0.015 |
RMSD bond angle | 1.800 * |
Data reduction software | TRUNCATE |
Data scaling software | CCP4 ((TRUNCATE)) |
Phasing software | SHARP |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.010 |
High resolution limit [Å] | 1.940 | 1.940 |
Rmerge | 0.048 * | 0.311 * |
Total number of observations | 86109 * | |
Number of reflections | 23095 * | |
<I/σ(I)> | 21.29 | 5.8 |
Completeness [%] | 98.8 * | 90.2 * |
Redundancy | 3.7 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 21 * | used microseeding * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | sodium acetate | 0.1 (M) | pH4.6 |
2 | 1 | reservoir | PEG4000 | 24 (%) | |
3 | 1 | reservoir | ammonium acetate | 0.2 (M) | |
4 | 1 | drop | protein | 6 (mg/ml) |