1N93

Crystal Structure of the Borna Disease Virus Nucleoprotein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE BW7B
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	BW7B
Temperature [K]	100
Detector technology	CCD
Collection date	2002-09-18
Detector	MARRESEARCH
Wavelength(s)	0.8841
Spacegroup name	I 4
Unit cell lengths	100.026, 100.026, 103.227
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.200 * - 1.760
R-factor	0.16421
Rwork	0.163
R-free	0.18700 *
Structure solution method	MAD
Starting model (for MR)	none
RMSD bond length	0.013
RMSD bond angle	1.340 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	REFMAC (5.1.19)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.700 *	1.810
High resolution limit [Å]	1.750	1.750
Rmerge	0.108 *	0.426 *
Number of reflections	50437	4392 *
<I/σ(I)>	28	3
Completeness [%]	98.6	86.5
Redundancy	4.8	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9	20 *	PEG 550 MME, NaCl, DTT, pH 9.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	14 (mg/ml)
2	1	reservoir	PEG550 MME	20 (%)
3	1	reservoir		0.1 (M)
4	1	reservoir	dithiothreitol	2 (mM)
5	1	reservoir	Bicine	0.1 (M)	pH9.0