1N93
Crystal Structure of the Borna Disease Virus Nucleoprotein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-09-18 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.8841 |
| Spacegroup name | I 4 |
| Unit cell lengths | 100.026, 100.026, 103.227 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.200 * - 1.760 |
| R-factor | 0.16421 |
| Rwork | 0.163 |
| R-free | 0.18700 * |
| Structure solution method | MAD |
| Starting model (for MR) | none |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.340 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.1.19) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.700 * | 1.810 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.108 * | 0.426 * |
| Number of reflections | 50437 | 4392 * |
| <I/σ(I)> | 28 | 3 |
| Completeness [%] | 98.6 | 86.5 |
| Redundancy | 4.8 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9 | 20 * | PEG 550 MME, NaCl, DTT, pH 9.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 14 (mg/ml) | |
| 2 | 1 | reservoir | PEG550 MME | 20 (%) | |
| 3 | 1 | reservoir | 0.1 (M) | ||
| 4 | 1 | reservoir | dithiothreitol | 2 (mM) | |
| 5 | 1 | reservoir | Bicine | 0.1 (M) | pH9.0 |
