1N8O
Crystal structure of a complex between bovine chymotrypsin and ecotin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 277 |
| Detector technology | IMAGE PLATE |
| Collection date | 1993-10-10 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 95.500, 63.800, 79.600 |
| Unit cell angles | 90.00, 91.70, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.000 |
| R-factor | 0.205 |
| Rwork | 0.205 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Bovine Chymotrypsin Ecotin |
| RMSD bond length | 0.002 |
| RMSD bond angle | 1.890 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | AMoRE |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.065 | 0.270 |
| Number of reflections | 30343 | |
| Completeness [%] | 91.0 | 67 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 293 | Ammonium sulfate 1.3M, Sodium Acetate 0.1M, 1% PEG 200, pH 4.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
