1N8E

Fragment Double-D from Human Fibrin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-1
Synchrotron site	SSRL
Beamline	BL9-1
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1999-02-02
Detector	MARRESEARCH
Wavelength(s)	0.98
Spacegroup name	P 21 21 21
Unit cell lengths	50.000, 125.350, 312.060
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 * - 4.500
R-factor	0.415
Rwork	0.415
R-free	0.41600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	fragment D of 1FZC
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	4.600
High resolution limit [Å]	4.500	4.500
Rmerge	0.138
Total number of observations	71273 *
Number of reflections	10214
Completeness [%]	83.4	53.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7 *	295	PEG-3350, 50 mM Tris, 7.5 mM CaCl2, VAPOR DIFFUSION, SITTING DROP, temperature 295K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	Tris	50 (mM)	pH7.0
3	1	drop		5 (mM)
4	1	drop	PEG3350	13.25 (%)
5	1	reservoir		10 (mM)
6	1	reservoir	Tris	50 (mM)	pH8.0