1N8B
Bacteriophage T4 baseplate structural protein gp8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-11-27 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 65.203, 65.766, 79.647 |
| Unit cell angles | 93.04, 100.32, 91.93 |
Refinement procedure
| Resolution | 30.980 - 2.900 |
| R-factor | 0.223635 |
| Rwork | 0.222 |
| R-free | 0.30000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1n7z |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.058 | 0.158 |
| Number of reflections | 28530 | |
| <I/σ(I)> | 22.2 | 6.3 |
| Completeness [%] | 99.1 | 98.3 |
| Redundancy | 1.9 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293.15 | HEPES, PEG 6000, LIBR, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.15K |
