1N86
Crystal structure of human D-dimer from cross-linked fibrin complexed with GPR and GHRPLDK peptide ligands.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-09-12 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 52.328, 130.093, 298.313 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.200 |
| R-factor | 0.23 |
| Rwork | 0.226 |
| R-free | 0.28900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | fragment D from 1FZC |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.460 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.170 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.131 | 0.564 |
| Total number of observations | 156847 * | |
| Number of reflections | 30589 | |
| Completeness [%] | 79.8 | 50 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 * | 295 | PEG 3350, Tris buffer. 10 mM CaCl2, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | double-D preparations | 9 (mg/ml) | |
| 2 | 1 | drop | Tris | 0.05 (M) | pH7.0 |
| 3 | 1 | drop | 5 (mM) | ||
| 4 | 1 | reservoir | PEG3350 | 12 (%) | |
| 5 | 1 | reservoir | 10 (mM) | ||
| 6 | 1 | reservoir | Tris | 50 (mM) | pH8.0 |
