1N82
The high-resolution crystal structure of IXT6, a thermophilic, intracellular xylanase from G. stearothermophilus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X26C |
Synchrotron site | NSLS |
Beamline | X26C |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2001-11-11 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.1000 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 169.481, 80.579, 79.054 |
Unit cell angles | 90.00, 91.89, 90.00 |
Refinement procedure
Resolution | 15.000 - 1.450 |
Rwork | 0.159 |
R-free | 0.19400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | initial model of Xylanase T-6 (family 10) of Bacillus stearothermophilus. |
RMSD bond length | 0.012 |
RMSD bond angle | 0.029 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.000 | 1.480 |
High resolution limit [Å] | 1.450 | 1.450 |
Number of reflections | 171850 | |
<I/σ(I)> | 18.1 | 1.5 |
Completeness [%] | 91.6 | 52.3 |
Redundancy | 7 | 5.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.1M sodium cacodylate pH=6.5, 1.9M sodium acetate , VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |