1N81
Crystal structure of Pfg27 from Plasmodium falciparum
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ELETTRA BEAMLINE 5.2R |
Synchrotron site | ELETTRA |
Beamline | 5.2R |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-10-13 |
Detector | MARRESEARCH |
Wavelength(s) | 1.0 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 58.880, 113.190, 91.600 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.000 * - 2.100 |
R-factor | 0.207 |
Rwork | 0.207 |
R-free | 0.25800 |
Structure solution method | MAD |
RMSD bond length | 0.011 * |
RMSD bond angle | 1.510 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 * | 15.000 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.039 | 0.282 |
Number of reflections | 18053 | |
<I/σ(I)> | 37.3 | 3.4 |
Completeness [%] | 99.0 | 99 |
Redundancy | 4 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 4 * | Hendrickson, W.A., (1990) EMBO J., 9, 1665. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | glucose | 0.2 (%(w/v)) | |
2 | 1 | reservoir | carbenicillin | 0.050 (mg/ml) |