1N80
Bacteriophage T4 baseplate structural protein gp8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-11-28 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0723 |
| Spacegroup name | P 1 |
| Unit cell lengths | 66.869, 66.777, 82.158 |
| Unit cell angles | 93.11, 101.28, 90.77 |
Refinement procedure
| Resolution | 39.110 - 2.450 |
| R-factor | 0.236 |
| Rwork | 0.236 |
| R-free | 0.28400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1n7z |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.360 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.490 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Rmerge | 0.053 | 0.176 |
| Number of reflections | 49960 | |
| <I/σ(I)> | 19.1 | 9.1 |
| Completeness [%] | 97.7 | 82.4 |
| Redundancy | 3.8 | 2.9 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 293.15 | HEPES, PEG 6000, LiCl, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.15K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 10 (mM) | pH7.5 |
| 3 | 1 | drop | dithiothreitol | 1 (mM) | |
| 4 | 1 | reservoir | 1 (M) | ||
| 5 | 1 | reservoir | Zwittergent-3-10 | 12 (mM) | |
| 6 | 1 | reservoir | HEPES | 0.1 (M) | pH7.0 |
| 7 | 1 | reservoir | PEG6000 | 16 (%) |
