1N7Z
Structure and location of gene product 8 in the bacteriophage T4 baseplate
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-D |
| Synchrotron site | APS |
| Beamline | 14-BM-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-12-15 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9797, 0.9799,0.9426 |
| Spacegroup name | P 1 |
| Unit cell lengths | 66.388, 78.472, 88.227 |
| Unit cell angles | 110.96, 97.60, 111.39 |
Refinement procedure
| Resolution | 40.000 * - 2.000 |
| R-factor | 0.213692 |
| Rwork | 0.213 |
| R-free | 0.24000 |
| Structure solution method | MAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.370 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELX |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.070 | 0.297 |
| Number of reflections | 97218 | |
| <I/σ(I)> | 15.1 | 4.2 |
| Completeness [%] | 97.5 | 96.5 |
| Redundancy | 1.9 | 1.8 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 293.15 | HEPES, PEG 6000, LiCl, DTT, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.15K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 10 (mM) | pH7.5 |
| 3 | 1 | drop | dithiothreitol | 1 (mM) | |
| 4 | 1 | reservoir | 1.5 (M) | ||
| 5 | 1 | reservoir | dithiothreitol | 1 (mM) | |
| 6 | 1 | reservoir | HEPES | 0.1 (M) | pH7.0 |
| 7 | 1 | reservoir | PEG6000 | 16 (%) |
