1N7W
Crystal Structure of Human Serum Transferrin, N-Lobe L66W mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-02-14 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 45.183, 57.916, 135.513 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 * - 2.100* |
| R-factor | 0.196 |
| Rwork | 0.196 |
| R-free | 0.23800 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1a8e |
| RMSD bond length | 0.008 * |
| RMSD bond angle | 22.300 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.180 |
| High resolution limit [Å] | 2.100 * | 2.100 |
| Rmerge | 0.093 * | 0.302 |
| Number of reflections | 21203 | 2018 * |
| <I/σ(I)> | 17.5 | 6.1 |
| Completeness [%] | 98.4 | 96 |
| Redundancy | 3.8 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.7 | 293 | 0.2M POTASSIUM ACETATE, 20% PEG 3350, pH 7.7, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 35 (mg/ml) | |
| 2 | 1 | drop | ammonium bicarbonate | 0.1 (M) | |
| 3 | 1 | reservoir | potassium acetate | 0.2 (M) | pH7.7 |
| 4 | 1 | reservoir | PEG3350 | 20 (%) |
