1N7V
THE RECEPTOR-BINDING PROTEIN P2 OF BACTERIOPHAGE PRD1: CRYSTAL FORM III
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-01-01 |
| Detector | BRANDEIS |
| Wavelength(s) | 0.95 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 46.500, 270.200, 137.200 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.110 - 2.200 |
| R-factor | 0.229 |
| Rwork | 0.229 |
| R-free | 0.22800 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | P2 Crystal Form I(1N7U) |
| RMSD bond length | 0.006 |
| RMSD bond angle | 26.500 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.000 | 2.340 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.064 * | 0.771 * |
| Number of reflections | 38750 | |
| <I/σ(I)> | 16.5 | |
| Completeness [%] | 87.4 | 58.7 * |
| Redundancy | 9.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 5 | 4 * | used macroseeding * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | MPD | 50 (%) | |
| 2 | 1 | reservoir | sodium acetate | 0.1 (M) | pH5.0 |
| 3 | 1 | reservoir | 10 (mM) |
