1N7E

Crystal structure of the sixth PDZ domain of GRIP1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X8C
Synchrotron site	NSLS
Beamline	X8C
Temperature [K]	110
Detector technology	CCD
Collection date	2000-09-21
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9
Spacegroup name	P 65 2 2
Unit cell lengths	40.267, 40.267, 222.872
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	15.000 * - 1.500
R-factor	0.254
Rwork	0.254
R-free	0.27800
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005 *
RMSD bond angle	1.200 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	1.590
High resolution limit [Å]	1.500	1.500
Rmerge	0.045 *	0.360
Number of reflections	151976
<I/σ(I)>	11.5	3.6
Completeness [%]	98.3	93.3
Redundancy	7.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	293	Park, S.H., (2002) Acta Cryst., D58, 1063. *