1N77
Crystal structure of Thermus thermophilus glutamyl-tRNA synthetase complexed with tRNA(Glu) and ATP.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-06-23 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 110.689, 219.124, 135.215 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 * - 2.400 |
| R-factor | 0.213 |
| Rwork | 0.213 |
| R-free | 0.25700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB 1G59 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 21.300 * |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.510 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.072 | 0.357 |
| Total number of observations | 247929 * | |
| Number of reflections | 59840 | |
| Completeness [%] | 91.2 | 80.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 6.5 * | 4 or 20 * | PEG1500, pH 6.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG1500 | ||
| 2 | 1 | 2 | PEG1500 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5.0 (mg/ml) | |
| 2 | 1 | drop | MOPS/Na | 10 (mM) | pH6.5 |
| 3 | 1 | drop | 5 (mM) | ||
| 4 | 1 | drop | 2-mercaptoethanol | 2.5 (mM) | |
| 5 | 1 | drop | PEG6000 | 1 (%) | |
| 6 | 1 | drop | ATP | 2 (mM) | |
| 7 | 1 | drop | glutamate | 2 (mM) | |
| 8 | 1 | reservoir | PEG | 10 (%) |
