1N76
CRYSTAL STRUCTURE OF HUMAN SEMINAL LACTOFERRIN AT 3.4 A RESOLUTION
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-10-13 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 56.052, 97.507, 156.790 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.650 - 3.400 |
| R-factor | 0.21937 |
| Rwork | 0.217 |
| R-free | 0.26599 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1b0l |
| RMSD bond length | 0.035 |
| RMSD bond angle | 2.061 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.520 |
| High resolution limit [Å] | 3.400 | 3.400 |
| Number of reflections | 11008 | |
| <I/σ(I)> | 6.4 | 1.9 |
| Completeness [%] | 93.8 | 75 |
| Redundancy | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICRODIALYSIS | 8 | 298 | Tris-HCl, ethanol, pH 8.0, MICRODIALYSIS, temperature 298.0K |
