1N73
Fibrin D-Dimer, Lamprey complexed with the PEPTIDE LIGAND: GLY-HIS-ARG-PRO-AMIDE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-04-23 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 |
| Unit cell lengths | 49.732, 99.302, 120.732 |
| Unit cell angles | 99.58, 101.46, 92.36 |
Refinement procedure
| Resolution | 15.000 * - 2.900 |
| R-factor | 0.26 |
| Rwork | 0.258 |
| R-free | 0.30800 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.410 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.084 | |
| Total number of observations | 75259 * | |
| Number of reflections | 40004 * | |
| Completeness [%] | 81.7 | 66.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 295 | PEG 3350 Tris buffer 5 mM CaCl2 2 mM GHRPam, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | lamprey double-D | 6-8 (mg/ml) | |
| 2 | 1 | drop | Tris | 50 (mM) | pH7.0 |
| 3 | 1 | drop | GHRPam | 4 (mM) | |
| 4 | 1 | drop | 5 (mM) | ||
| 5 | 1 | reservoir | PEG | 5.5 (%) | |
| 6 | 1 | reservoir | Tris | 50 (mM) | pH7.0 |
