1N6K
Crystal Structure of Human Rab5a A30P mutant complex with GDP and aluminum fluoride
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2002-09-03 |
Detector | MARRESEARCH |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 35.840, 64.040, 65.880 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.550 |
R-factor | 0.18 |
Rwork | 0.177 |
R-free | 0.20000 |
Structure solution method | FOURIER SYNTHESIS |
RMSD bond length | 0.011 |
RMSD bond angle | 1.560 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.610 |
High resolution limit [Å] | 1.550 | 1.550 |
Rmerge | 0.049 | 0.350 |
Total number of observations | 116591 * | |
Number of reflections | 22654 * | |
<I/σ(I)> | 31 | 3.7 |
Completeness [%] | 99.8 * | 99.4 * |
Redundancy | 5.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 298 | PEG 6000, sodium chloride, MES, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 15 (mg/ml) | |
2 | 1 | drop | Tris-HCl | 20 (mM) | pH8.0 |
3 | 1 | drop | GppNHp | 5 (mM) | |
4 | 1 | reservoir | PEG6000 | 10 (%(w/v)) | |
5 | 1 | reservoir | MES | 50-100 (mM) | pH6.0 |
6 | 1 | reservoir | 0.2 (M) | ||
7 | 1 | reservoir | 1 (mM) | ||
8 | 1 | reservoir | beta-mercaptoethanol | 0.1 (%(v/v)) |