1N67
Clumping Factor A from Staphylococcus aureus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X9B |
| Synchrotron site | NSLS |
| Beamline | X9B |
| Temperature [K] | 180 |
| Detector technology | CCD |
| Collection date | 1999-08-05 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9176 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 38.700, 80.540, 113.890 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.280 - 1.900 |
| R-factor | 0.206 |
| Rwork | 0.206 |
| R-free | 0.25670 * |
| Structure solution method | MIR |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.370 * |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.280 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.044 | 0.284 |
| Total number of observations | 148963 * | |
| Number of reflections | 28604 | |
| <I/σ(I)> | 15.7 | 5 |
| Completeness [%] | 99.0 * | 95.2 |
| Redundancy | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 295.5 | Sodium citrate, Succinic acid, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 22.5K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein/peptide mixture | 14 (mg/ml) | |
| 2 | 1 | reservoir | ammonium acetate | 200 (mM) | |
| 3 | 1 | reservoir | sodium citrate dihydrate | 100 (mM) | pH5.6 |
| 4 | 1 | reservoir | PEG4000 | 26 (%(w/v)) |
