1N5Z
Complex structure of Pex13p SH3 domain with a peptide of Pex14p
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X31 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X31 |
| Temperature [K] | 200 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-12-10 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 50.350, 63.204, 66.935 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.700 |
| R-factor | 0.24568 |
| Rwork | 0.244 |
| R-free | 0.30800 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 * |
| RMSD bond angle | 1.310 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | BEAST |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.065 * | 0.330 * |
| Number of reflections | 5907 | |
| <I/σ(I)> | 21.1 | 2.9 |
| Completeness [%] | 93.8 | 97.4 |
| Redundancy | 4.3 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7.3 | 22 * | Ammonium sulfate, lithium sulfate, tris, pH 7.3, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Pex13p(SH3) | 1.25 (mM) | |
| 2 | 1 | drop | peptide | 5 (mM) | |
| 3 | 1 | reservoir | ammonium sulfate | 2.0 (M) | |
| 4 | 1 | reservoir | Tris | 100 (mM) | pH7.3 |
| 5 | 1 | reservoir | lithium sulfate | 200 (mM) |
