Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
Synchrotron site | MPG/DESY, HAMBURG |
Beamline | BW6 |
Temperature [K] | 110 |
Detector technology | IMAGE PLATE |
Collection date | 1999-07-14 |
Detector | MARRESEARCH |
Wavelength(s) | 1.05 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 119.295, 132.088, 159.825 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 18.000 - 1.500 |
R-factor | 0.135 |
Rwork | 0.135 |
R-free | 0.16900 * |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.017 * |
RMSD bond angle | 1.946 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.520 |
High resolution limit [Å] | 1.490 | 1.490 |
Rmerge | 0.046 * | |
Total number of observations | 941420 * | |
Number of reflections | 387014 | |
Completeness [%] | 95.9 | 65.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 7.3 | 279 | Dobbek, H., (1999) Proc.Natl.Acad.Sci.USA, 96, 8884. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | 1 | 0.8 (M) | ||
2 | 1 | 1 | 0.8 (M) | ||
3 | 1 | 1 | MPD | 2 (%) | |
4 | 1 | 1 | HEPES | 100 (mM) | pH7.3 |