Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-05-27 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.98 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 52.550, 92.670, 101.050 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.540 - 2.000 |
R-factor | 0.248 |
Rwork | 0.248 |
R-free | 0.29000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1k6z |
RMSD bond length | 0.006 |
RMSD bond angle | 1.100 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP ((CCP4)) |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.720 | 2.010 |
High resolution limit [Å] | 1.980 | 1.980 |
Rmerge | 0.037 | 0.235 |
Number of reflections | 34752 | |
<I/σ(I)> | 30.6 | 5 |
Completeness [%] | 89.2 | |
Redundancy | 4.4 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.7 | 291 | PEG 400, ammonium sulphate, sodium chloride, hepes, TCEP, pH 7.7, VAPOR DIFFUSION, HANGING DROP, temperature 291K |