1N5A
Crystal structure of the Murine class I Major Histocompatibility Complex of H-2DB, B2-Microglobulin, and A 9-Residue immunodominant peptide epitope gp33 derived from LCMV
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I711 |
| Synchrotron site | MAX II |
| Beamline | I711 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-09-28 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.0292 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 91.998, 122.657, 99.180 |
| Unit cell angles | 90.00, 103.34, 90.00 |
Refinement procedure
| Resolution | 20.000 * - 2.850 |
| R-factor | 0.25264 |
| Rwork | 0.251 |
| R-free | 0.27900 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hoc |
| RMSD bond length | 0.019 * |
| RMSD bond angle | 1.920 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.900 |
| High resolution limit [Å] | 2.850 | 2.850 |
| Rmerge | 0.096 * | 0.254 * |
| Total number of observations | 135354 * | |
| Number of reflections | 46560 | |
| <I/σ(I)> | 12 | 9.8 |
| Completeness [%] | 92.6 | 98 * |
| Redundancy | 2.9 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 * | 298 | Ammonium sulfate, ethylene glycol, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | ammonium sulfate | 1.8 (M) | |
| 2 | 1 | reservoir | Tris-Cl | 0.1 (M) | pH8.5 |
| 3 | 1 | reservoir | ethylene glycol | 3-4 (%) | |
| 4 | 1 | drop | protein | 6 (mg/ml) | |
| 5 | 1 | drop | Tris-Cl | 20 (mM) | pH7.0 |
