1N54
Cap Binding Complex m7GpppG free
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2002-01-27 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.943 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 111.770, 111.770, 175.730 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.000 * - 2.750* |
| Rwork | 0.263 |
| R-free | 0.29100 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1n52 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 21.500 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 * | 2.850 |
| High resolution limit [Å] | 2.750 * | 2.690 |
| Rmerge | 0.061 * | |
| Number of reflections | 39454 * | |
| <I/σ(I)> | 20 | 2.3 |
| Completeness [%] | 100.0 * | |
| Redundancy | 11 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.25 | 4 * | Peg 400, Magnesium chloride, glycerol, Tris, glycine, ethylene glycol, pH 7.25, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 12 (mg/ml) | |
| 2 | 1 | reservoir | PEG400 | 22 (%(w/v)) | |
| 3 | 1 | reservoir | Tris-HCl | 100 (mM) | pH7.25 |
| 4 | 1 | reservoir | 125 (mM) | ||
| 5 | 1 | reservoir | glycerol | 10 (%(v/v)) | |
| 6 | 1 | reservoir | dithiothreitol | 2 (mM) | |
| 7 | 1 | reservoir | glycine | 10 (mM) | |
| 8 | 1 | reservoir | ethylene glycol | 3 (%(v/v)) |
