1N51
Aminopeptidase P in complex with the inhibitor apstatin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2000-10-24 |
Detector | RIGAKU RAXIS IIC |
Spacegroup name | I 41 2 2 |
Unit cell lengths | 139.319, 139.319, 231.005 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 * - 2.300 |
Rwork | 0.179 |
R-free | 0.20400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB 1AZ9 |
RMSD bond length | 0.005 |
RMSD bond angle | 1.200 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 * | 2.280 |
High resolution limit [Å] | 2.300 * | 2.200 |
Rmerge | 0.044 * | 0.411 * |
Number of reflections | 55327 | |
<I/σ(I)> | 16.58 | 1.46 |
Completeness [%] | 97.0 * | 96.2 * |
Redundancy | 2.6 * | 2.4 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 277 | Magnesium Acetate, Sodium Cacodylate, MPD, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 8.7 (mg/ml) | |
2 | 1 | reservoir | magnesium acetate | 0.2 (M) | |
3 | 1 | reservoir | sodium cacodylate | 0.1 (M) | pH6.8 |
4 | 1 | reservoir | MPD | 25 (%) |