1N4V
ATOMIC RESOLUTION STRUCTURE OF CHOLESTEROL OXIDASE @pH 5.8 (STREPTOMYCES SP. SA-COO)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X8C |
| Synchrotron site | NSLS |
| Beamline | X8C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-04-23 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.354, 73.291, 63.171 |
| Unit cell angles | 90.00, 105.10, 90.00 |
Refinement procedure
| Resolution | 46.900 - 1.000 |
| Rwork | 0.119 |
| R-free | 0.14680 |
| Structure solution method | AB INITIO PHASING |
| Starting model (for MR) | 1mxt |
| RMSD bond length | 0.015 |
| RMSD bond angle | 0.030 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELX |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.900 | 1.020 |
| High resolution limit [Å] | 1.000 | 1.000 |
| Rmerge | 0.069 | 0.656 |
| Number of reflections | 230865 | |
| <I/σ(I)> | 7.2 | 1.7 |
| Completeness [%] | 95.0 | 90.8 |
| Redundancy | 4.1 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.8 | PEG 8000, MANGANESE SULFATE, CACODYLATE, pH 5.8 |
