1N4Q

Protein Geranylgeranyltransferase type-I Complexed with a GGPP Analog and a KKKSKTKCVIL Peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-C
Synchrotron site	APS
Beamline	14-BM-C
Temperature [K]	100
Detector technology	CCD
Collection date	2001-03-17
Detector	ADSC QUANTUM 4
Wavelength(s)	1.00000
Spacegroup name	C 1 2 1
Unit cell lengths	271.055, 268.033, 184.971
Unit cell angles	90.00, 131.73, 90.00

Refinement procedure

Resolution	29.990 - 2.400
R-factor	0.214
Rwork	0.214
R-free	0.23400
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.190 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000 *	2.490
High resolution limit [Å]	2.400	2.400
Rmerge	0.056 *	0.352 *
Total number of observations	997257 *
Number of reflections	355317 *
<I/σ(I)>	14	2.1
Completeness [%]	93.0 *	87.1
Redundancy	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.3	17 *	ammonium sulfate, sodium citrate, dithiothreitol, pH 6.3, VAPOR DIFFUSION, HANGING DROP, temperature 290K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15 (mg/ml)
2	1	reservoir	ammonium sulfate	1.3 (M)
3	1	reservoir		175 (mM)	pH6.5
4	1	reservoir	dithiothreitol	20 (mM)
5	1	reservoir	MES	100 (mM)	pH6.3