1N4O
Crystal structure of the Class A beta-lactamase L2 from Stenotrophomonas maltophilia
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LURE BEAMLINE DW32 |
| Synchrotron site | LURE |
| Beamline | DW32 |
| Temperature [K] | 283 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-05-25 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9485 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 135.503, 135.503, 94.856 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.850 |
| R-factor | 0.155 |
| Rwork | 0.147 |
| R-free | 0.16400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bza |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.753 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.950 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.064 | 0.286 |
| Number of reflections | 75048 | |
| <I/σ(I)> | 22.7 | 2.6 |
| Completeness [%] | 99.2 | 95.2 |
| Redundancy | 6.1 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | ammonium sulfate 1.5M, sodium cacodylate 0.1M, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
