1N4M

Structure of Rb tumor suppressor bound to the transactivation domain of E2F-2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 6B
Synchrotron site	PAL/PLS
Beamline	6B
Detector technology	IMAGE PLATE
Collection date	2002-06-02
Detector	MACSCIENCE
Wavelength(s)	1.12714
Spacegroup name	P 1
Unit cell lengths	54.369, 65.164, 69.339
Unit cell angles	85.55, 79.48, 67.16

Refinement procedure

Resolution	20.000 * - 2.200
R-factor	0.222
Rwork	0.222
R-free	0.28600 *
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.380 *
Data reduction software	DENZO
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.200	2.200 *
Rmerge	0.082 *	0.237 *
Total number of observations	93798 *
Number of reflections	37599
Completeness [%]	86.3 *	70.3 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5 *	4 *	Ammonium sulfate Citrate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	drop	Tris-HCl	25 (mM)	pH7.5
3	1	drop		200 (mM)
4	1	drop	dithiothreitol	5 (mM)
5	1	reservoir	ammonium sulfate	1.5 (M)
6	1	reservoir	sodium citrate	100 (mM)	pH5.5