1N4M
Structure of Rb tumor suppressor bound to the transactivation domain of E2F-2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 6B |
| Synchrotron site | PAL/PLS |
| Beamline | 6B |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-06-02 |
| Detector | MACSCIENCE |
| Wavelength(s) | 1.12714 |
| Spacegroup name | P 1 |
| Unit cell lengths | 54.369, 65.164, 69.339 |
| Unit cell angles | 85.55, 79.48, 67.16 |
Refinement procedure
| Resolution | 20.000 * - 2.200 |
| R-factor | 0.222 |
| Rwork | 0.222 |
| R-free | 0.28600 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.380 * |
| Data reduction software | DENZO |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | |
| High resolution limit [Å] | 2.200 | 2.200 * |
| Rmerge | 0.082 * | 0.237 * |
| Total number of observations | 93798 * | |
| Number of reflections | 37599 | |
| Completeness [%] | 86.3 * | 70.3 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 4 * | Ammonium sulfate Citrate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 25 (mM) | pH7.5 |
| 3 | 1 | drop | 200 (mM) | ||
| 4 | 1 | drop | dithiothreitol | 5 (mM) | |
| 5 | 1 | reservoir | ammonium sulfate | 1.5 (M) | |
| 6 | 1 | reservoir | sodium citrate | 100 (mM) | pH5.5 |
