1N4L
A DNA analogue of the polypurine tract of HIV-1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 108 |
Detector technology | CCD |
Collection date | 2002-02-02 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.1123 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 54.410, 146.222, 46.778 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.000 |
R-factor | 0.241 |
Rwork | 0.240 |
R-free | 0.26900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1d1u |
RMSD bond length | 0.006 |
RMSD bond angle | 1.300 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 * | 2.100 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.118 * | |
Number of reflections | 23122 | |
<I/σ(I)> | 5.8 | |
Completeness [%] | 93.9 * | 84.8 * |
Redundancy | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 4 * | PEG 4000, NaCl, ADA, MgCl2, HEPES, pH 6.5, VAPOR DIFFUSION, HANGING DROP at 277K |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 4 * | PEG 4000, NaCl, ADA, MgCl2, HEPES, pH 6.5, VAPOR DIFFUSION, HANGING DROP at 277K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | NaCl | ||
10 | 1 | 2 | PEG 4000 | ||
2 | 1 | 1 | MgCl2 | ||
3 | 1 | 1 | ADA | ||
4 | 1 | 1 | HEPES | ||
5 | 1 | 1 | PEG 4000 | ||
6 | 1 | 2 | NaCl | ||
7 | 1 | 2 | MgCl2 | ||
8 | 1 | 2 | ADA | ||
9 | 1 | 2 | HEPES |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 0.4 (mM) | |
2 | 1 | drop | oligonucleotide | 0.5 (mM) | |
3 | 1 | reservoir | PEG4000 | 8 (%(w/v)) | |
4 | 1 | reservoir | ADA | 0.05 (M) | pH6.5 |
5 | 1 | reservoir | magnesium acetate | 5.0 (mM) |