1N4J
STREPTAVIDIN MUTANT N23A AT 2.18A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | SIEMENS |
Temperature [K] | 293 |
Detector technology | AREA DETECTOR |
Collection date | 1996-07-03 |
Detector | XENTRONICS |
Wavelength(s) | 1.5418 |
Spacegroup name | I 41 2 2 |
Unit cell lengths | 59.200, 59.200, 179.800 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 2.200 * |
Rwork | 0.188 |
R-free | 0.25300 * |
Structure solution method | isomorphous |
Starting model (for MR) | 1rst |
RMSD bond length | 0.005 |
RMSD bond angle | 0.022 |
Data reduction software | SAINT |
Data scaling software | SAINT |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 10.000 * | |
High resolution limit [Å] | 2.200 * | |
Rmerge | 0.070 | 0.250 * |
Number of reflections | 8077 * | |
Completeness [%] | 86.0 | 62 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 6.2 | 293 | PEG4000, phosphate buffer, pH 6.2, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 20-30 (mg/ml) | |
2 | 1 | reservoir | PEG4000 | 10 (%) | |
3 | 1 | reservoir | phosphate | 0.1 (M) | pH6.2 |