1N4J

STREPTAVIDIN MUTANT N23A AT 2.18A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	SIEMENS
Temperature [K]	293
Detector technology	AREA DETECTOR
Collection date	1996-07-03
Detector	XENTRONICS
Wavelength(s)	1.5418
Spacegroup name	I 41 2 2
Unit cell lengths	59.200, 59.200, 179.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.200 *
Rwork	0.188
R-free	0.25300 *
Structure solution method	isomorphous
Starting model (for MR)	1rst
RMSD bond length	0.005
RMSD bond angle	0.022
Data reduction software	SAINT
Data scaling software	SAINT
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	10.000 *
High resolution limit [Å]	2.200 *
Rmerge	0.070	0.250 *
Number of reflections	8077 *
Completeness [%]	86.0	62 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.2	293	PEG4000, phosphate buffer, pH 6.2, VAPOR DIFFUSION, SITTING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20-30 (mg/ml)
2	1	reservoir	PEG4000	10 (%)
3	1	reservoir	phosphate	0.1 (M)	pH6.2