1N44

Crystal Structure of Annexin V R23E Mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	293
Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS IIC
Spacegroup name	H 3
Unit cell lengths	157.757, 157.757, 36.761
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 3.000
R-factor	0.1631
Rwork	0.163
R-free	0.24120
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	1.528
RMSD bond angle	0.010
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.200
High resolution limit [Å]	2.700	3.040
Rmerge	0.126 *	0.234 *
Number of reflections	8460
Completeness [%]	93.8 *	89.8 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	8.2	290	ammonium sulfate, CaCl2, pH 8.2, VAPOR DIFFUSION, SITTING DROP, temperature 290K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium sulfate	34-40 (%sat)
2	1	reservoir	HEPES	50 (mM)	pH8.2
3	1	reservoir	dithiothreitol	2 (mM)
4	1	reservoir		10 (mM)
5	1	drop	protein	14 (mg/ml)
6	1	drop		20 (mM)