1N3W

Engineered High-Affinity Maltose-Binding Protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2000-12-07
Detector	MARRESEARCH
Wavelength(s)	1.54179
Spacegroup name	P 43 21 2
Unit cell lengths	121.510, 121.510, 61.380
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.400 * - 2.600
R-factor	0.244
Rwork	0.244
R-free	0.28600 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1anf
RMSD bond length	0.007 *
RMSD bond angle	22.700 *
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.390	2.690
High resolution limit [Å]	2.600	2.590
Rmerge	0.065	0.196 *
Number of reflections	16214
<I/σ(I)>	30.1
Completeness [%]	99.8 *	99.9 *
Redundancy	4.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	290	PEG 4000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 290K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	cacodylate	10 (mM)	pH6.5
2	1	reservoir		200 (mM)
3	1	reservoir	zinc acetate	18 (mM)
4	1	reservoir	PEG8000	20 (%)