1N38
reovirus polymerase lambda3 elongation complex with one phosphodiester bond formed
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F1 |
Synchrotron site | CHESS |
Beamline | F1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2000-12-20 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.948 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 70.719, 85.207, 248.473 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 * - 2.800 |
R-factor | 0.201 |
Rwork | 0.201 |
R-free | 0.26000 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | lambda3 native structure |
RMSD bond length | 0.007 * |
RMSD bond angle | 1.394 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 * | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.139 | 0.522 |
Number of reflections | 33254 | |
<I/σ(I)> | 10 | 2 |
Completeness [%] | 86.4 | 82.1 |
Redundancy | 5-6 * | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 22 * | PEG4000, NaCl, HEPES, glycerol, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | PEG4000 | ||
2 | 1 | 1 | NaCl | ||
3 | 1 | 1 | HEPES | ||
4 | 1 | 1 | glycerol | ||
5 | 1 | 2 | PEG4000 | ||
6 | 1 | 2 | NaCl | ||
7 | 1 | 2 | HEPES | ||
8 | 1 | 2 | glycerol |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 8.7 (mg/ml) | |
2 | 1 | drop | sodium HEPES | 50 (mM) | pH7.8 |
3 | 1 | drop | glycerol | 10 (%) | |
4 | 1 | drop | 150 (mM) | ||
5 | 1 | drop | dithiothreitol | 10 (mM) | |
6 | 1 | drop | 0.02 (%) | ||
7 | 1 | reservoir | PEG8000 | 7.5 (%) | |
8 | 1 | reservoir | 10 (%) | ||
9 | 1 | reservoir | sodium HEPES | 0.1 (M) | pH7.8 |