1N38
reovirus polymerase lambda3 elongation complex with one phosphodiester bond formed
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-12-20 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.948 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 70.719, 85.207, 248.473 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 * - 2.800 |
| R-factor | 0.201 |
| Rwork | 0.201 |
| R-free | 0.26000 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | lambda3 native structure |
| RMSD bond length | 0.007 * |
| RMSD bond angle | 1.394 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 * | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.139 | 0.522 |
| Number of reflections | 33254 | |
| <I/σ(I)> | 10 | 2 |
| Completeness [%] | 86.4 | 82.1 |
| Redundancy | 5-6 * | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 22 * | PEG4000, NaCl, HEPES, glycerol, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG4000 | ||
| 2 | 1 | 1 | NaCl | ||
| 3 | 1 | 1 | HEPES | ||
| 4 | 1 | 1 | glycerol | ||
| 5 | 1 | 2 | PEG4000 | ||
| 6 | 1 | 2 | NaCl | ||
| 7 | 1 | 2 | HEPES | ||
| 8 | 1 | 2 | glycerol |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 8.7 (mg/ml) | |
| 2 | 1 | drop | sodium HEPES | 50 (mM) | pH7.8 |
| 3 | 1 | drop | glycerol | 10 (%) | |
| 4 | 1 | drop | 150 (mM) | ||
| 5 | 1 | drop | dithiothreitol | 10 (mM) | |
| 6 | 1 | drop | 0.02 (%) | ||
| 7 | 1 | reservoir | PEG8000 | 7.5 (%) | |
| 8 | 1 | reservoir | 10 (%) | ||
| 9 | 1 | reservoir | sodium HEPES | 0.1 (M) | pH7.8 |
