1N2D
Ternary complex of MLC1P bound to IQ2 and IQ3 of Myo2p, a class V myosin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-08-01 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 80.017, 64.240, 72.785 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.270 - 2.000 |
| Rwork | 0.208 |
| R-free | 0.25700 |
| Structure solution method | MAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 19.000 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SnB |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.270 | 2.010 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.098 | 0.423 |
| Number of reflections | 25861 | |
| <I/σ(I)> | 17 | 5 |
| Completeness [%] | 99.5 | 99.1 |
| Redundancy | 63.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 293 | Terrak, M., (2002) Acta Crystallogr.,Sect.D, 58, 1882. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | sodium acetate | 5 (mM) | pH4.0 |
| 2 | 1 | drop | 1 (mM) | ||
| 3 | 1 | drop | dithiothreitol | 1 (mM) | |
| 4 | 1 | drop | protein | 10 (mg/ml) | |
| 5 | 1 | reservoir | PEG5000 MME | 23 (%) | |
| 6 | 1 | reservoir | potassium fluoride | 0.3 (M) |
