1N29

Crystal structure of the N1A mutant of human group IIA phospholipase A2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	ENRAF-NONIUS
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2000-03-21
Detector	MARRESEARCH
Wavelength(s)	1.5418
Spacegroup name	P 61 2 2
Unit cell lengths	74.790, 74.790, 88.891
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	15.000 - 2.600
Rwork	0.242
R-free	0.31970
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	2.800
High resolution limit [Å]	2.600	2.600
Rmerge	0.066 *
Total number of observations	31180 *
Number of reflections	4693 *
Completeness [%]	99.5 *	99.8
Redundancy	3.4 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7.5	4 *	NaCl, CaCl2, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	Ca2+	5 (mM)
3	1	drop	Tris-HCl	0.1 (M)	pH7.5
4	1	drop		5.5 (M)
5	1	drop	octyl-beta-glucoside	0.5 (mM)
6	1	reservoir	PEG550 MME