1N25
Crystal structure of the SV40 Large T antigen helicase domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-06-20 |
Detector | SBC-2 |
Wavelength(s) | 1.074385 |
Spacegroup name | P 3 2 1 |
Unit cell lengths | 120.321, 120.321, 132.202 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.000 - 2.800 |
Rwork | 0.243 |
R-free | 0.27500 * |
Structure solution method | MAD |
RMSD bond length | 0.010 * |
RMSD bond angle | 1.379 * |
Data reduction software | TRUNCATE |
Data scaling software | CCP4 ((TRUNCATE)) |
Phasing software | SOLVE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.125 | 0.544 |
Total number of observations | 171257 * | |
Number of reflections | 27610 | |
<I/σ(I)> | 16.5 | 3.9 |
Completeness [%] | 98.4 | 99.2 |
Redundancy | 6.2 | 5.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 4 * | glycerol, DTT, Hepes, NaCl, EtOH, EDTA, pH 7.6, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 20 (mg/ml) | |
10 | 1 | reservoir | EDTA | 1 (mM) | |
2 | 1 | drop | Tris-HCl | 25 (mM) | pH8.0 |
3 | 1 | drop | 250 (mM) | ||
4 | 1 | drop | EDTA | 1 (mM) | |
5 | 1 | drop | dithiothreitol | 10 (mM) | |
6 | 1 | reservoir | dithiothreitol | 20 (mM) | |
7 | 1 | reservoir | HEPES | 50 (mM) | pH7.6 |
8 | 1 | reservoir | 105 (mM) | ||
9 | 1 | reservoir | ethanol | 5 (%) |