1N1J

Crystal structure of the NF-YB/NF-YC histone pair

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM30A
Synchrotron site	ESRF
Beamline	BM30A
Temperature [K]	100
Detector technology	CCD
Collection date	2001-11-18
Detector	MARRESEARCH
Wavelength(s)	0.920023
Spacegroup name	P 21 21 21
Unit cell lengths	51.624, 60.345, 61.645
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.000 - 1.670
Rwork	0.181
R-free	0.20600
Structure solution method	MAD
RMSD bond length	0.006
RMSD bond angle	19.500 *
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	24.000 *	1.710
High resolution limit [Å]	1.670	1.670
Rmerge	0.026 *	0.053 *
Total number of observations	110854 *
Number of reflections	22726
<I/σ(I)>	43.8	17.3
Completeness [%]	97.9 *	99 *
Redundancy	4.9	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7.5	298	PEG 4000, magnesium acetate, sodium hepes, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10-14 (mg/ml)
2	1	reservoir	sodium HEPES	0.1 (M)	pH7.5
3	1	reservoir		0.2 (M)
4	1	reservoir	PEG4000	10-14 (%)