1N1J
Crystal structure of the NF-YB/NF-YC histone pair
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM30A |
Synchrotron site | ESRF |
Beamline | BM30A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-11-18 |
Detector | MARRESEARCH |
Wavelength(s) | 0.920023 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 51.624, 60.345, 61.645 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 24.000 - 1.670 |
Rwork | 0.181 |
R-free | 0.20600 |
Structure solution method | MAD |
RMSD bond length | 0.006 |
RMSD bond angle | 19.500 * |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 24.000 * | 1.710 |
High resolution limit [Å] | 1.670 | 1.670 |
Rmerge | 0.026 * | 0.053 * |
Total number of observations | 110854 * | |
Number of reflections | 22726 | |
<I/σ(I)> | 43.8 | 17.3 |
Completeness [%] | 97.9 * | 99 * |
Redundancy | 4.9 | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 7.5 | 298 | PEG 4000, magnesium acetate, sodium hepes, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10-14 (mg/ml) | |
2 | 1 | reservoir | sodium HEPES | 0.1 (M) | pH7.5 |
3 | 1 | reservoir | 0.2 (M) | ||
4 | 1 | reservoir | PEG4000 | 10-14 (%) |