1N1D
Glycerol-3-phosphate cytidylyltransferase complexed with CDP-glycerol
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 140 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-12-10 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 |
| Unit cell lengths | 37.795, 55.929, 63.701 |
| Unit cell angles | 88.99, 75.03, 82.54 |
Refinement procedure
| Resolution | 15.000 - 1.800 * |
| R-factor | 0.2463 |
| Rwork | 0.241 |
| R-free | 0.29440 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1coz |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.000 | 1.870 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.051 * | 0.191 * |
| Total number of observations | 121437 * | |
| Number of reflections | 40610 * | |
| <I/σ(I)> | 20.6 | 5.7 |
| Completeness [%] | 87.8 | 82.1 |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 8.5 | 298 | PEG 3350, lithium sulfate, Tris, EDTA, DTT, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | Tris | 100 (mM) | pH8.5 |
| 3 | 1 | drop | EDTA | 1 (mM) | |
| 4 | 1 | drop | dithiothreitol | 1 (mM) | |
| 5 | 1 | drop | CDP-glycerol | 5-10 (mM) | |
| 6 | 1 | reservoir | PEG3350 | 30 (%) | |
| 7 | 1 | reservoir | 200 (mM) | ||
| 8 | 1 | reservoir | Tris | 100 (mM) | pH8.5 |
