1N19
Structure of the HSOD A4V mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL7-1 |
Synchrotron site | SSRL |
Beamline | BL7-1 |
Temperature [K] | 90 |
Detector technology | IMAGE PLATE |
Collection date | 1999-08-11 |
Detector | MARRESEARCH |
Wavelength(s) | 1.08 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 114.700, 47.900, 56.200 |
Unit cell angles | 90.00, 96.80, 90.00 |
Refinement procedure
Resolution | 27.950 * - 1.860 |
R-factor | 0.207 |
Rwork | 0.207 |
R-free | 0.25900 * |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 24.900 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 27.950 * | 1.830 |
High resolution limit [Å] | 1.860 * | 1.860 * |
Rmerge | 0.054 * | 0.283 * |
Total number of observations | 187289 * | |
Number of reflections | 23358 * | |
<I/σ(I)> | 17.7 | 1.57 |
Completeness [%] | 84.1 * | 57.9 * |
Redundancy | 3.3 | 2.6 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Microdialysis * | 8 * | 293 | ammonium sulfate, NaCl, Tris, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | 1 | 60-900 (mM) | ||
2 | 1 | 1 | Tris | 50 (mM) | pH8. |
3 | 1 | 2 | Tris | 20 (mM) | pH8.5 |