1N11
D34 REGION OF HUMAN ANKYRIN-R AND LINKER
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-01-31 |
| Detector | SBC-2 |
| Wavelength(s) | 0.97925 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 137.329, 137.329, 197.801 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.700 |
| R-factor | 0.318 |
| Rwork | 0.319 |
| R-free | 0.30300 |
| Structure solution method | MAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.620 |
| Data reduction software | TRUNCATE |
| Data scaling software | CCP4 ((TRUNCATE)) |
| Phasing software | MLPHARE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.540 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.082 | 0.699 |
| Total number of observations | 415031 * | |
| Number of reflections | 37994 * | |
| <I/σ(I)> | 22.6 | 1.3 |
| Completeness [%] | 98.4 | 93 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7.5 | 4 * | PEG400, HEPES, NaBr, CaCl2, acetonitrile, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 25 (mg/ml) | |
| 2 | 1 | drop | HEPES-Na | 20 (mM) | pH7.5 |
| 3 | 1 | drop | 500 (mM) | ||
| 4 | 1 | drop | EDTA | 1 (mM) | |
| 5 | 1 | drop | dithiothreitol | 1 (mM) | |
| 6 | 1 | reservoir | HEPES | 100 (mM) | pH7.5 |
| 7 | 1 | reservoir | 500 (mM) | ||
| 8 | 1 | reservoir | PEG400 | 7-10 (%(v/v)) | |
| 9 | 1 | reservoir | acetonitrile | 5 (%(v/v)) |
