1N0S
ENGINEERED LIPOCALIN FLUA IN COMPLEX WITH FLUORESCEIN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Detector technology | AREA DETECTOR |
| Collection date | 2001-05-15 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.797, 85.039, 66.059 |
| Unit cell angles | 90.00, 106.18, 90.00 |
Refinement procedure
| Resolution | 99.000 * - 2.000 |
| R-factor | 0.19562 |
| Rwork | 0.193 |
| R-free | 0.24300 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1kxo |
| RMSD bond length | 0.015 |
| RMSD bond angle | 2.671 |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR |
| Refinement software | REFMAC (5.1.19) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.050 * | 0.470 * |
| Total number of observations | 79526 * | |
| Number of reflections | 29552 | |
| <I/σ(I)> | 17.64 | 2.73 |
| Completeness [%] | 95.8 | 96.1 |
| Redundancy | 5.39 | 2.73 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.1 | 298 | 2.0 M (NH4)2SO4, 2% (w/v) PEG 400, 10 mM hepes/naoh, pH 8.1, VAPOR DIFFUSION, HANGING DROP, temperature 298 KK |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 23 (mg/ml) | |
| 2 | 1 | drop | fluorescein | 1 (mM) | |
| 3 | 1 | reservoir | ammonium sulfate | 2.0 (M) | |
| 4 | 1 | reservoir | PEG400 | 2 (%(w/v)) | |
| 5 | 1 | reservoir | HEPES-NaOH | 10 (mM) | pH8.1 |
