1N0R

4ANK: A designed ankyrin repeat protein with four identical consensus repeats

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	77
Detector technology	CCD
Collection date	2002-01-29
Detector	ADSC QUANTUM 210
Wavelength(s)	0.9199
Spacegroup name	P 21 21 21
Unit cell lengths	28.429, 46.115, 81.588
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.650 - 1.500
R-factor	0.21763
Rwork	0.216
R-free	0.24900 *
Structure solution method	Single anomolous diffraction
RMSD bond length	0.011
RMSD bond angle	1.490 *
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SOLVE
Refinement software	REFMAC (5.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.140
High resolution limit [Å]	1.500
Rmerge	0.070 *	0.436 *
Number of reflections	16966	913 *
Completeness [%]	99.9 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.4	293	sodium cacodylate, magnesium bromide, MPD, isopropanol, pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	reservoir		0.2 (M)
3	1	reservoir	sodium cacodylate	0.1 (M)	pH6.4
4	1	reservoir	MPD	33 (%)
5	1	reservoir	isopropanol	15 (%)