1N0Q

3ANK: A designed ankyrin repeat protein with three identical consensus repeats

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	77
Detector technology	CCD
Collection date	2002-03-15
Detector	ADSC QUANTUM 210
Wavelength(s)	1.000
Spacegroup name	P 21 21 21
Unit cell lengths	39.636, 43.159, 105.574
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.960 - 1.260
R-factor	0.17218
Rwork	0.171
R-free	0.18700 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ank
RMSD bond length	0.011
RMSD bond angle	1.410 *
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	EPMR
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.960
High resolution limit [Å]	1.260
Rmerge	0.087 *	0.080 *
Number of reflections	41302	2246 *
<I/σ(I)>	11.8
Completeness [%]	99.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	HEPES, MPD, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	reservoir	HEPES	0.1 (M)	pH7.0
3	1	reservoir	MPD	50 (%)
4	1	drop	MPD	30 (%)