1N0L
Crystal structure of the PapD chaperone (C-terminally 6x histidine-tagged) bound to the PapE pilus subunit (N-terminal-deleted) from uropathogenic E. coli
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 98 |
Detector technology | IMAGE PLATE |
Detector | RIGAKU RAXIS IV |
Spacegroup name | P 1 |
Unit cell lengths | 56.070, 56.863, 60.931 |
Unit cell angles | 108.57, 89.26, 104.14 |
Refinement procedure
Resolution | 30.000 * - 2.300 |
R-factor | 0.226 |
Rwork | 0.226 |
R-free | 0.27900 |
Structure solution method | MAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.290 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | |
High resolution limit [Å] | 2.300 | |
Rmerge | 0.041 * | 0.175 * |
Total number of observations | 162110 * | |
Number of reflections | 27884 | |
<I/σ(I)> | 28.7 | |
Completeness [%] | 91.0 | 91.5 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 294 | 100 mM Tris pH 8.5, 250 mM MgCl2, 17-18% PEG 4000, 4-7% glycerol, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | Tris | 100 (mM) | pH8.5 |
2 | 1 | reservoir | 250 (mM) | ||
3 | 1 | reservoir | PEG4000 | 17-18 (%) | |
4 | 1 | reservoir | glycerol | 4-7 (%) |