1N0G

Crystal Structure of A Cell Division and Cell Wall Biosynthesis Protein UPF0040 from Mycoplasma pneumoniae: Indication of A Novel Fold with A Possible New Conserved Sequence Motif

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	CCD
Collection date	2001-10-17
Detector	ADSC QUANTUM 4
Wavelength(s)	1.0
Spacegroup name	P 4
Unit cell lengths	101.442, 101.442, 38.193
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 * - 2.800
Rwork	0.229
R-free	0.27800 *
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	22.900 *
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000 *	2.850
High resolution limit [Å]	2.800	2.800
Rmerge	0.046 *	0.285 *
Number of reflections	9888	486 *
<I/σ(I)>	22.4	3.5
Completeness [%]	99.9 *	100
Redundancy	6.8	6.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5 *	20 *	VAPOR DIFFUSION, HANGING DROP

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	19 (mg/ml)
2	1	drop		0.5 (M)
3	1	drop		0.2 (M)
4	1	drop	Tris-HCl	0.1 (M)	pH8.5
5	1	drop	PEG3350	18 (%(w/v))
6	1	drop	glycerol	20 (%)